V/Line is a statutory authority that operates regional passenger train and coach services in the Australian state of Victoria. It provides passenger train services on five commuter lines and eight long-distance routes from its major hub at Southern Cross railway station in Melbourne. It also provides bus services across Victoria and into New South Wales, the Australian Capital Territory and South Australia. In addition, V/Line is responsible for the maintenance of much of the Victorian freight and passenger rail network outside of the areas managed by Metro Trains Melbourne and the Australian Rail Track Corporation.

Regional rail is a term used for passenger rail services that operate between towns and cities. These trains operate with more stops than inter-city rail, and unlike commuter rail, operate beyond the limits of urban areas, connecting smaller cities and towns.