West Virginia Route 28 is a north–south route through the Potomac Highlands of the U.S. state of West Virginia. The southern terminus of the route is at West Virginia Route 39 in Huntersville. The northern terminus is at the Maryland state line in Wiley Ford, where the route continues into Cumberland as Canal Parkway upon crossing the North Branch Potomac River.

The North Branch Potomac River flows from Fairfax Stone in West Virginia to its confluence with the South Branch Potomac River near Green Spring, West Virginia, where it turns into the Potomac River proper.