The 16"/45 caliber gun was used for the main batteries of the last class of Standard-type battleships for the United States Navy, the Colorado-class. These guns promised twice the muzzle energy over the Mark 7 12-inch/50 caliber guns of the Wyoming-class battleship and a 50% increase over the 14-inch/45 caliber guns of the New York-class, Nevada-class, and Pennsylvania-class battleships.

The Colorado-class battleships were a group of four United States Navy super-dreadnoughts, the last of its pre-Treaty battleships. Designed during World War I, their construction overlapped the end of that conflict and continued in its immediate aftermath. Though all four keels were laid, only three ships entered service: Colorado, Maryland, and West Virginia. Washington was over 75% completed when she was canceled under the terms of the Washington Naval Treaty in 1922. As such, the 16" gun Colorado-class ships were the last and most powerful battleships built by the U.S. Navy until the North Carolina class entered service on the eve of World War II.