The 1853 Wisconsin gubernatorial election was held on November 4, 1853. Democratic candidate William A. Barstow won the election with 55% of the vote, winning his first term as Governor of Wisconsin. Barstow defeated Free Soil Party candidate Edward D. Holton and Whig candidate Henry S. Baird. This would be the last Wisconsin gubernatorial election in which there was a Whig candidate on the ballot.

William Augustus Barstow was an American businessman, politician, and public administrator. He was the third governor and second Secretary of State of Wisconsin, and served as a Union Army officer during the American Civil War. Before Wisconsin became a state, he was instrumental in the creation of Waukesha County.