The 1899 Sewanee Tigers football team represented Sewanee: The University of the South in the 1899 Southern Intercollegiate Athletic Association football season. Sewanee was one of the first college football powers of the South and the 1899 team was one of its best. The 1899 Tigers won 12 games and lost none, outscored opponents 322–10, and won the Southern Intercollegiate Athletic Association (SIAA) title.

The University of the South, familiarly known as Sewanee, is a private Episcopal liberal arts college in Sewanee, Tennessee. It is owned by 28 southern dioceses of the Episcopal Church, and its School of Theology is an official seminary of the church. The university's School of Letters offers graduate degrees in American Literature and Creative Writing. The campus consists of 13,000 acres (53 km2) of scenic mountain property atop the Cumberland Plateau, with the developed portion occupying about 1,000 acres (4.0 km2).