The 1900 Western University of Pennsylvania football team was an American football team that represented Western University of Pennsylvania as an independent during the 1900 college football season.

M. Roy "Slab" Jackson was an early professional football player who played for the Duquesne Country and Athletic Club. He also served as the team's captain and coach in 1898–1900. Jackson, a fullback, scored 14 touchdowns, often on short plunges after teammates J. A. Gammons or Dave Fultz had put the ball close to the end zone. In 1898, Jackson played for Duquesne against the Western Pennsylvania All-Stars in the first pro football all-star game. During the game, he would score two of the Duquesnes touchdowns.