The 1940 Florida gubernatorial election was held on November 5, 1940. Incumbent Governor Fred P. Cone was term-limited. Democratic nominee Spessard Holland was elected unopposed.

Spessard Lindsey Holland was an American lawyer and politician. He served as the 28th Governor of Florida from 1941 to 1945, and later as a US senator for Florida from 1946 to 1971. He was the first person born in Florida to serve as Governor and US Senator for the state. While serving as a US Senator he would notably introduce the 24th Amendment.