The 1959 First 500 Mile NASCAR International Sweepstakes at Daytona was the second race of the 1959 NASCAR Grand National Series season. It was held on February 22, 1959, in front of 41,921 spectators. It was the first race held at the 2.5-mile Daytona International Speedway.

The Daytona 500 is a 500-mile-long (805 km) NASCAR Cup Series motor race held annually at Daytona International Speedway in Daytona Beach, Florida. It is the first of two Cup races held every year at Daytona, the second being the Coke Zero Sugar 400, and one of three held in Florida, with the annual fall showdown Dixie Vodka 400 being held at Homestead south of Miami. From 1988 to 2019, it was one of the four restrictor plate races on the Cup schedule. The inaugural Daytona 500 was held in 1959 coinciding with the opening of the speedway and since 1982, it has been the season-opening race of the Cup series.