The 1965 Giro d'Italia was the 48th running of the Giro d'Italia, one of cycling's Grand Tour races. The Giro started in San Marino, on 15 May, with a 295 km (183.3 mi) stage and concluded in Florence, on 6 June, with a 136 km (84.5 mi) leg. A total of 100 riders from 10 teams entered the 22-stage race, which was won by Italian Vittorio Adorni of the Salvarani team. The second and third places were taken by Italian riders Italo Zilioli and Felice Gimondi, respectively.

Vittorio Adorni was an Italian professional road racing cyclist.