The 1965 Tour de France was the 52nd edition of the Tour de France, one of cycling's Grand Tours. It took place between 22 June and 14 July, with 22 stages covering a distance of 4,188 km (2,602 mi). In his first year as a professional, Felice Gimondi, a substitute replacement on the Salvarani team, captured the overall title ahead of Raymond Poulidor, the previous year's second-place finisher.

Felice Gimondi was an Italian professional racing cyclist. With his 1968 victory at the Vuelta a España, only three years after becoming a professional cyclist, Gimondi, nicknamed "The Phoenix", was the second cyclist to win all three Grand Tours of road cycling: Tour de France, Giro d'Italia, and Vuelta a España (1968). He is one of only seven cyclists to have done so.