The 1965 Tour de France was the 52nd edition of the Tour de France, one of cycling's Grand Tours. It took place between 22 June and 14 July, with 22 stages covering a distance of 4,188 km (2,602 mi). In his first year as a professional, Felice Gimondi, a substitute replacement on the Salvarani team, captured the overall title ahead of Raymond Poulidor, the previous year's second-place finisher.

The mountains classification is a secondary competition in the Tour de France, that started in 1933. It is given to the rider that gains the most points for reaching mountain summits first. The leader of the classification is named the King of the Mountains, and since 1975 wears the polka dot jersey, a white jersey with red polka dots.