The 1989 Football League Third Division play-off final was a two-legged football match played on 31 May and 3 June 1989, between Port Vale and Bristol Rovers to determine the third and final team to gain promotion from the Third Division to the Second Division. The top two teams of the 1988–89 Football League Third Division season gained automatic promotion to the Second Division, while those placed from third to sixth place in the table took part in play-offs; the winners of these semi-finals competed for the final place for the 1989–90 season in the Second Division. From 1990 onwards, play-off finals would be one-legged affairs decided at Wembley Stadium, or an appropriate neutral stadium.

Port Vale Football Club are a professional football club based in Burslem, Stoke-on-Trent, England, which compete in EFL League One but will play in EFL League Two in the 2024–25 season after suffering relegation. Vale are named after the valley of ports on the Trent and Mersey Canal. They have never played top-flight football, and hold the record for the most seasons in the English Football League (112) without reaching the first tier. After playing at the Athletic Ground in Cobridge and The Old Recreation Ground in Hanley, the club returned to Burslem when Vale Park was opened in 1950. Outside the ground is a statue to Roy Sproson, who played 842 competitive games for the club. The club's traditional rivals are Stoke City, and games between the two are known as the Potteries derby.