The 1991 Madrilenian regional election was held on Sunday, 26 May 1991, to elect the 3rd Assembly of the Autonomous Community of Madrid. All 101 seats in the Assembly were up for election. The election was held simultaneously with regional elections in twelve other autonomous communities and local elections all throughout Spain.

The Assembly of Madrid or Madrid Assembly is the unicameral autonomous legislature of the Autonomous Community of Madrid since the approval of the Madrid Charter of Autonomy in 1983.