The 2000–01 Azadegan League was the tenth and last season of the Azadegan League as first tier of Iran Football league that was won by Esteghlal.

The Azadegan League, also known as League 1, is the second highest division of professional football in Iran. It was the top-level football league in Iran from its foundation in 1991 until 2001, when the Persian Gulf Pro League was established.