The 2000 Football League Third Division play-off Final was an association football match which was played on 26 May 2000 at Wembley Stadium, London, between Peterborough United and Darlington. It was to determine the fourth and final team to gain promotion from the Football League Third Division to the Second Division. The top three teams of the 1999–2000 Football League Third Division, Swansea City, Rotherham United and Northampton Town, gained automatic promotion to the Second Division, while those placed from fourth to seventh place in the table took part in play-offs. The winners of the play-off semi-finals competed for the final place for the 2000–01 season in the Second Division. The losing semi-finalists were Hartlepool United and Barnet who had been defeated by Darlington and Peterborough United respectively.

Peterborough United Football Club is a professional association football club based in Peterborough, Cambridgeshire, England. The team compete in League One, the third level of the English football league system.