The 2000 United States Senate election in Florida was held on November 7, 2000, on the same date as the U.S. House of Representatives and presidential election. Incumbent Republican Senator Connie Mack III decided to retire instead of seeking a third term. Democrat Bill Nelson won the open seat, even as Republican presidential nominee George W. Bush narrowly triumphed over Al Gore in the state by a mere 537 votes. Bill McCollum's 46.19% popular vote percentage is the highest for a losing Republican United States Senate candidate in Florida.

Ira William McCollum Jr. is an American lawyer and Republican Party politician. He was a member of the United States House of Representatives from 1981 to 2001, representing Florida's 5th congressional district, which was later redistricted to the 8th congressional district in 1993. As a member of the House, McCollum rose to become Vice Chairman of the House Republican Conference, the fifth-highest ranking position in the House Republican leadership. He voted to impeach President Bill Clinton and subsequently took a leadership role in managing Clinton's trial in the Senate, which ended in acquittal.