The 2006 12 Hours of Sebring was the 54th running of this event, and took place on March 18, 2006. The race was sponsored by Mobil 1 and was the opening race of the 2006 American Le Mans Series season run by IMSA.

The 12 Hours of Sebring is an annual motorsport endurance race for sports cars held at Sebring International Raceway, on the site of the former Hendricks Army Airfield World War II air base in Sebring, Florida, US. In the past, this race has been a round of the now defunct World Sportscar Championship, IMSA GT Championship and American Le Mans Series. In 2012, the race was the opening event of the FIA World Endurance Championship in a one off race before being returned back to the American Le Mans Series for 2013. Starting in 2014, the event became the second round of the WeatherTech SportsCar Championship.