The 2007 Mobil 1 12 Hours of Sebring was the 55th running of this event and the opening round of the 2007 American Le Mans Series that took place on March 17, 2007.

The 12 Hours of Sebring is an annual motorsport endurance race for sports cars held at Sebring International Raceway, on the site of the former Hendricks Army Airfield World War II air base in Sebring, Florida, US. In the past, this race has been a round of the now defunct World Sportscar Championship, IMSA GT Championship and American Le Mans Series. In 2012, the race was the opening event of the FIA World Endurance Championship in a one off race before being returned back to the American Le Mans Series for 2013. Starting in 2014, the event became the second round of the WeatherTech SportsCar Championship.