The group stage featured 32 teams: the 22 automatic qualifiers and the 10 winners of the play-off round.

Football Club Zenit, also known as Zenit Saint Petersburg or simply Zenit, is a Russian professional football club based in Saint Petersburg. Founded in 1925, the club plays in the Russian Premier League. Zenit are the reigning champions of the Russian Premier League. Previously they won the 2007, 2010, 2011–12, 2014–15, 2018–19, 2019–20, 2020–21 and the 2021–22 seasons of the Russian Premier League, as well as the 2007–08 UEFA Cup and the 2008 UEFA Super Cup. The club is owned and sponsored by the Russian state-owned energy giant Gazprom. The team play its home matches at the Gazprom Arena. In March 2022, the club was expelled from all European and international club competitions by FIFA and the UEFA due to the Russian invasion of Ukraine. In addition, the European Club Association suspended the team. On 24 May 2023, Zenit became the first Russian sports club with 10 million followers on social media.