The 2016 EFL League One play-off final was an association football match which was played on 29 May 2016 at Wembley Stadium, London, between Barnsley and Millwall to determine the third and final team to gain promotion from EFL League One to the EFL Championship. The top two teams of the 2015–16 Football League One season gained automatic promotion to the Championship, while the teams placed from third to sixth place in the table partook in play-off semi-finals; the winners of these semi-finals competed for the final place for the 2016–17 season in the Championship.

Barnsley Football Club is a professional football club in Barnsley, South Yorkshire, England, which competes in EFL League One, the third level of the English football league system.