The tenth edition of the Strade Bianche road cycling race was held on 5 March 2016, in Tuscany, Italy. Swiss Fabian Cancellara concluded his third win in the race. The race covered 176 km (109 mi), starting and finishing in Siena. It was part of the 2016 UCI Europe Tour, a 1.HC-ranked event.

The Strade Bianche is a road bicycle race in Tuscany, Central Italy, starting and finishing in Siena. First held in 2007, it is raced annually on the first or second Saturday of March. The name Strade Bianche stems from the historic white gravel roads in the Crete Senesi, which are a defining feature of the race. One-third of the total race distance is raced on dirt roads, covering 63 km (39 mi) of strade bianche, spread over 11 sectors.