The 2016 United States Senate election in South Dakota was held November 8, 2016, to elect a member of the United States Senate to represent the State of South Dakota, concurrently with the 2016 U.S. presidential election, as well as other elections to the United States Senate in other states and elections to the United States House of Representatives and various state and local elections. The primaries were held June 7.

John Randolph Thune is an American politician serving as the senior United States senator from South Dakota, a seat he has held since 2005. Thune is in his fourth Senate term and is the Senate minority whip, a post he has held since 2021. A member of the Republican Party, Thune served three terms as the U.S. representative for South Dakota's at-large congressional district from 1997 to 2003.