The 2016 United States Senate election in Wisconsin was held November 8, 2016, to elect a member of the United States Senate to represent the State of Wisconsin, concurrently with the 2016 U.S. presidential election, as well as other elections to the United States Senate in other states and elections to the United States House of Representatives and various state and local elections. The primaries were held August 9, 2016.

Ronald Harold Johnson is an American politician serving as the senior United States senator from Wisconsin, a seat he has held since 2011. A Republican, Johnson was first elected to the U.S. Senate in 2010, defeating Democratic incumbent Russ Feingold. He was reelected in 2016, defeating Feingold in a rematch, and in 2022, narrowly defeating Lieutenant Governor Mandela Barnes.