The 21st GLAAD Media Awards was the 2010 annual presentation of the media awards presented by the Gay & Lesbian Alliance Against Defamation. The awards seek to honor films, television shows, musicians and works of journalism that fairly and accurately represent the LGBT community and issues relevant to the community. The 21st annual award ceremony included 116 nominees in 24 English-language categories, and 36 Spanish-language nominees in eight categories.

The GLAAD Media Award is a US accolade bestowed by GLAAD to recognize and honor various branches of the media for their outstanding representations of the lesbian, gay, bisexual and transgender (LGBT) community and the issues that affect their lives. In addition to film and television, the Awards also recognize achievements in other branches of the media and arts, including theatre, music, journalism and advertising.