The 527th Space Aggressor Squadron is a United States Space Force unit assigned to the Space Training and Readiness Command. The unit traces its lineage to the 312th Bombardment Squadron (Light) constituted in 1942. It presents realistic adversary threats to US and allied military forces to improve their training for space-associated operations. It is stationed at Schriever Space Force Base, Colorado. Its present form dates from its activation as part of the United States Air Force in 2000. That year it was activated as part of the Space Warfare Centre, but it was then transferred to the 57th Adversary Tactics Group in 2006. The mission was augmented with the activation of a United States Air Force Reserve associate unit, the 26th Space Aggressor Squadron, on 1 October 2003. With the formation of the Space Force in 2019, the squadron was part of the second wave of transfers and reorganizations which took place in mid-2020. It was realigned under Space Delta 11 upon that organizations activation in 2021.

Space Training and Readiness Command is the United States Space Force's education, training, doctrine, and test field command. It is headquartered at Peterson Space Force Base, Colorado.