The 59th Medical Wing (MDW) is the U.S. Air Force's largest medical wing and is the Air Force functional medical command for Joint Base San Antonio (JBSA). It comprises seven medical groups across San Antonio. Three are located at the Wilford Hall Ambulatory Surgical Center (WHASC); the 959th Medical Group is located at San Antonio Military Medical Center (SAMMC), JBSA-Fort Sam Houston; the 59th Training Group - the wing's newest group, activated on 4 January 2016, is also located at JBSA-Fort Sam Houston. The 359th and 559th Medical Groups are located at and support the missions of JBSA-Randolph and JBSA-Lackland, respectively.

Brooke Army Medical Center (BAMC) is the United States Army's premier medical institution. Located on Fort Sam Houston, BAMC, a 425-bed Academic Medical Center, is the Department of Defense's largest facility and only Level 1 Trauma Center. BAMC is also home to the Center for the Intrepid, an outpatient rehabilitation facility. The center is composed of ten separate organizations, including community medical clinics, centered around the Army's largest in-patient hospital. BAMC is staffed by more than 8,000 Soldiers, Airmen, Sailors, Civilians, and Contractors providing care to wounded Service Members and the San Antonio Community at-large.