Affinity electrophoresis
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Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electropho
The quantitative principle of affinity electrophoresis illustrated with electrophoresis at pH 8.6 of concanavalin A into an agarose gel containing blood serum (3.6 microliter per square cm). The bar indicates 1 cm. Electrophoresis performed overnight at less than 10 V/cm. The analysis was performed early in the 1970s at the Protein Laboratory
Lectin
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Lectins are carbohydrate-binding proteins that are highly specific for sugar groups that are part of sugars and other molecules. Lectins can recognize specific types of sugar moieties and play a role
Affinity electrophoresis
…fragments may be characterized by their affinity to other molecules. The methods have been used for estimation of binding constants, as for instance in lectin affinity electrophoresis or characterization of molecules with specific features like glycan content or ligand binding. For enzymes and other ligand-binding…
A typical legume lectin monomer (lentil lectin), complexed with a sugar (glucose). The four sugar-binding loops are shown in different colours. The variable loop that confers monosaccharide specificity is shown in orange.