Agnus Dei is an oil painting completed between 1635 and 1640 by the Spanish Baroque artist Francisco de Zurbarán. It is housed in the Prado Museum in Madrid, Spain. The Lamb of God is an allusion to Christ's title as recorded in John's Gospel, where John the Baptist describes Jesus as "The Lamb of God who takes away the sin of the World".

Francisco de Zurbarán was a Spanish painter. He is known primarily for his religious paintings depicting monks, nuns, and martyrs, and for his still-lifes. Zurbarán gained the nickname "Spanish Caravaggio", owing to the forceful use of chiaroscuro in which he excelled.