Al-Zaytuna Mosque, also known as Ez-Zitouna Mosque, and El-Zituna Mosque, is a major mosque at the center of the Medina of Tunis in Tunis, Tunisia. The mosque is the oldest in the city and covers an area of 5,000 square metres with nine entrances. It was founded at the end of the 7th century or in the early 8th century, but its current architectural form dates from a reconstruction in the 9th century, including many antique columns reused from Carthage, and from later additions and restorations over the centuries. The mosque hosted one of the first and greatest universities in the history of Islam. Many Muslim scholars graduated from al-Zaytuna for over a thousand years. Ibn 'Arafa, a major Maliki scholar, al-Maziri, the great traditionalist and jurist, and Aboul-Qacem Echebbi, a famous Tunisian poet, all taught there, among others.

Tunis is the capital and largest city of Tunisia. The greater metropolitan area of Tunis, often referred to as "Grand Tunis", has about 2,700,000 inhabitants. As of 2020, it is the third-largest city in the Maghreb region and the eleventh-largest in the Arab world.