Alachua is the second-largest city in Alachua County, Florida and the third-largest in North Central Florida. According to the 2020 census, the city's population was 10,574, up from 9,059 at the 2010 census. It is part of the Gainesville, Florida Metropolitan Statistical Area. Alachua has one of the largest bio and life sciences sectors in Florida and is the site for the Santa Fe College Perry Center for Emerging Technologies.

Alachua County is a county in the north central portion of the U.S. state of Florida. As of the 2020 census, the population was 278,468. The county seat is Gainesville, the home of the University of Florida since 1906, when the campus opened with 106 students.