Alberta Provincial Highway No. 16, commonly referred to as Highway 16, is a major east–west highway in central Alberta, Canada, connecting Jasper to Lloydminster via Edmonton. It forms a portion of the Yellowhead Highway, a major interprovincial route of the Trans-Canada Highway system that stretches from Masset, British Columbia, to Portage la Prairie, Manitoba, near Winnipeg. Highway 16 spans approximately 634 km (394 mi) from Alberta's border with British Columbia in the west to its border with Saskatchewan in the east. As of 2010, all but less than 96 km (60 mi) of the route was divided, with a minimum of two lanes in each direction. It is designated a core route in Canada's National Highway System.

Jasper is a specialized municipality and townsite in western Alberta within the Canadian Rockies. The townsite is in the Athabasca River valley and is the commercial centre of Jasper National Park.