The Alfred I. duPont Testamentary Trust is a non-profit organization created by philanthropist Alfred Irénée du Pont in 1935, devoted to supporting the trust's sole charitable beneficiary, the Nemours Foundation. As of 2015, the organization stated it oversaw approximately $5 billion in assets.

Alfred Irénée du Pont was an American industrialist, financier, philanthropist and a member of the influential Du Pont family.