Altoona is an unincorporated community and census-designated place (CDP) in Lake County, Florida, United States. The population was 89 at the 2010 census. It is part of the Orlando–Kissimmee–Sanford Metropolitan Statistical Area.

Lake County is a county in the central portion of the U.S. state of Florida. As of the 2020 census, the population was 383,956. Its county seat is Tavares, and its largest city is Clermont. Lake County is included in the Orlando–Kissimmee–Sanford Metropolitan Statistical Area.