Amesville is a village in Athens County, Ohio, United States, located on Federal Creek. The population was 171 at the 2020 census.

Athens County is a county in southeastern Ohio. As of the 2020 census, the population was 62,431. Its county seat and largest city is Athens. The county was formed in 1805 from Washington County. Because Ohio's first state university, Ohio University, was established here in 1804, the town and the county are named for the ancient center of learning, Athens, Greece.