Amityville is the westernmost station on the Babylon Branch of the Long Island Rail Road in Suffolk County. It is located on John Street in Amityville, New York, but the official description of its location is not as precise. The MTA describes the station as being located on John Street between Sunrise Highway and NY 27A west of NY 110. John Street is located between Sterling Place and West Oak Street.

The Babylon Branch is a rail service operated by the Long Island Rail Road in the U.S. state of New York. The term refers to the trains serving Montauk Branch stations from Valley Stream east to Babylon; in other words, the Babylon Branch is a rail service rather than an actual track. The electrification of the Montauk Branch ends east of the Babylon station, so the Babylon Branch is mostly served by electric trains.