The women's 800 metres was the longest of the four women's track races in the Athletics at the 1964 Summer Olympics program in Tokyo. It was held on 18 October, 19 October, and 20 October 1964. 24 athletes from 16 nations entered, with 1 not starting the first round. The first round was held on 18 October, the semifinals on 19 October, and the final on 20 October.

At the 1964 Summer Olympics in Tokyo, the athletics competition included 36 events, 24 for men and 12 for women. The women's 400 metres and women's pentathlon events were newly introduced at these Games. There were a total number of 1016 participating athletes from 82 countries.