The Avenue U station is a local station on the IND Culver Line of the New York City Subway, located at the intersection of Avenue U and McDonald Avenue in Gravesend, Brooklyn. It is served by the F train at all times and the <F> train during rush hours in the peak direction.

The IND Culver Line is a rapid transit line of the B Division of the New York City Subway, extending from Downtown Brooklyn south to Coney Island, Brooklyn, New York City, United States. The local tracks of the Culver Line are served by the F service, as well as the G between Bergen Street and Church Avenue. The express tracks north of Church Avenue are used by the <F> train during rush hours in the peak direction. The peak-direction express track between Ditmas Avenue and Avenue X has not seen regular service since 1987.