Aylesbury railway station is a railway station in Aylesbury, Buckinghamshire, England, on the London–Aylesbury line from London Marylebone via Amersham. It is 38 miles (61 km) from Aylesbury to Marylebone. A branch line from Princes Risborough on the Chiltern Main Line terminates at the station. It was the terminus for London Underground's Metropolitan line until the service was cut back to Amersham in 1961. The station was also known as Aylesbury Town under the management of British Railways from c. 1948 until the 1960s.

Aylesbury is the county town of Buckinghamshire, South East England. It is home to the Roald Dahl Children's Gallery and the Waterside Theatre. It is located in central Buckinghamshire, midway between High Wycombe and Milton Keynes.