The Banbury to Verney Junction branch line was a railway branch line constructed by the Buckinghamshire Railway which connected the Oxfordshire market town of Banbury with the former Oxford/Cambridge Varsity line and the former Metropolitan Railway at Verney Junction, a distance of 21 miles 39 chains. Onward routes from there ran to the West Coast Main Line at Bletchley via Brackley and Buckingham and thence to Cambridge, or to Aylesbury for London.

The Varsity Line was the main railway line that linked the English university cities of Oxford and Cambridge, operated by the London and North Western Railway.