Basilica of Our Lady of the Immaculate Conception is a Roman Catholic minor basilica and parish church in Guelph, Ontario, Canada. A Gothic Revival style building constructed between 1876 and 1888 by architect Joseph Connolly, it is considered Connolly's best work. The monumental church contains decorative carving and stained glass executed by skilled craftsmen. The church of Our Lady is one of the 122 parishes in the Diocese of Hamilton and currently has 2,600 families in the congregation.

Guelph is a city in Southwestern Ontario, Canada. Known as The Royal City, it is roughly 22 km (14 mi) east of Kitchener and 70 km (43 mi) west of Downtown Toronto, at the intersection of Highway 6, Highway 7 and Wellington County Road 124. It is the seat of Wellington County, but is politically independent of it.