Basilius Besler (1561–1629) was a respected Nuremberg apothecary and botanist, best known for his monumental florilegium, the Hortus Eystettensis, 1613.

Hortus Eystettensis is the short title of a book produced by Basilius Besler, a Nuremberg apothecary and botanist, in 1613 describing the plants of the garden of the Prince-Bishop of Eichstätt in Bavaria. The Renaissance style garden was located at the bishop's palace at the Willibaldsburg and was created over eight terraces overlooking the city of Eichstätt. It was the first botanical garden in Germany, and the only one outside of Italy. The bishop had brought plants from all over the world for his garden, and it was thought that it contained examples of all of the shrubs and flowering plants known to horticulturalists at the time.