The Bendigo V/Line rail service is a regional passenger rail service operated by V/Line in Victoria, Australia. It serves passengers between state capital Melbourne, Victoria and the regional city of Bendigo, Victoria.

V/Line is a statutory authority that operates regional passenger train and coach services in the Australian state of Victoria. It provides passenger train services on five commuter lines and eight long-distance routes from its major hub at Southern Cross railway station in Melbourne. It also provides bus services across Victoria and into New South Wales, the Australian Capital Territory and South Australia. In addition, V/Line is responsible for the maintenance of much of the Victorian freight and passenger rail network outside of the areas managed by Metro Trains Melbourne and the Australian Rail Track Corporation.