Berkeley is a market town and civil parish in the Stroud District in Gloucestershire, England. It lies in the Vale of Berkeley between the east bank of the River Severn and the M5 motorway. The town is noted for Berkeley Castle, where the imprisoned King Edward II is believed to have been murdered, as well as the birthplace of the physician Edward Jenner, pioneer of the smallpox vaccine, the world's first vaccine. The parish includes the village of Berkeley Heath.

Stroud District is a local government district in Gloucestershire, England. The district is named after its largest town of Stroud. The council is based at Ebley Mill in Cainscross. The district also includes the towns of Berkeley, Dursley, Nailsworth, Stonehouse and Wotton-under-Edge, along with numerous villages and surrounding rural areas. Over half of the district lies within the Cotswolds Area of Outstanding Natural Beauty.