Berrysburg is a borough in Dauphin County, Pennsylvania, United States. The population was 324 at the 2020 census. It is part of the Harrisburg metropolitan area.

Pennsylvania Route 25 is a 35-mile-long (56 km) state highway located in Dauphin and Schuylkill counties in central Pennsylvania. The western terminus of the route is at U.S. Route 209 in Millersburg. The eastern terminus is at US 209 in Newtown. PA 25 runs parallel to the Mahantongo Mountains and the Mahantango Valley, as well as US 209, for its entire length. The route passes through the communities of Berrysburg, Gratz, Sacramento, and Hegins. PA 25 intersects PA 225 in Berrysburg, PA 125 in Hegins, and Interstate 81 (I-81) in Frailey Township. PA 25 was designated in 1928 to run from US 209 in Millersburg east to PA 443 in Pine Grove, running east to Hegins before heading southeast to Tremont and south to Pine Grove. The road between Hegins and Newtown was designated as PA 125. PA 25 was realigned to head east from Hegins to Newtown in the 1930s, with the former alignment between Pine Grove and Hegins becoming a relocated PA 125.