Biosafety is the prevention of large-scale loss of biological integrity, focusing both on ecology and human health. These prevention mechanisms include the conduction of regular reviews of biosafety in laboratory settings, as well as strict guidelines to follow. Biosafety is used to protect from harmful incidents. Many laboratories handling biohazards employ an ongoing risk management assessment and enforcement process for biosafety. Failures to follow such protocols can lead to increased risk of exposure to biohazards or pathogens. Human error and poor technique contribute to unnecessary exposure and compromise the best safeguards set into place for protection.

Biological integrity is associated with how "pristine" an environment is and its function relative to the potential or original state of an ecosystem before human alterations were imposed. Biological integrity is built on the assumption that a decline in the values of an ecosystem's functions are primarily caused by human activity or alterations. The more an environment and its original processes are altered, the less biological integrity it holds for the community as a whole. If these processes were to change over time naturally, without human influence, the integrity of the ecosystem would remain intact. The integrity of the ecosystem relies heavily on the processes that occur within it because those determine what organisms can inhabit an area and the complexities of their interactions. Most of the applications of the notion of biological integrity have addressed aquatic environments, but there have been efforts to apply the concept to terrestrial environments. Determining the pristine condition of the ecosystem is in theory scientifically derived, but deciding which of the many possible states or conditions of an ecosystem is the appropriate or desirable goal is a political or policy decision and is typically the focus of policy and political disagreements. Ecosystem health is a related concept but differs from biological integrity in that the "desired condition" of the ecosystem or environment is explicitly based on the values or priorities of society.