A blackwater river is a type of river with a slow-moving channel flowing through forested swamps or wetlands. Most major blackwater rivers are in the Amazon Basin and the Southern United States. The term is used in fluvial studies, geology, geography, ecology, and biology. Not all dark rivers are blackwater in that technical sense. Some rivers in temperate regions, which drain or flow through areas of dark black loam, are simply black due to the color of the soil; these rivers are black mud rivers. There are also black mud estuaries.

The Rio Negro, or Guainía as it is known in its upper part, is the largest left tributary of the Amazon River, the largest blackwater river in the world, and one of the world's ten largest rivers by average discharge.