Botanical Garden station is a commuter rail stop on the Metro-North Railroad's Harlem Line, serving the Bedford Park section of the Bronx, New York City. The station is located just north of the intersection of Southern Boulevard and Bedford Park Boulevard adjacent to northern Bronx Park and the New York Botanical Garden. The station has two high-level side platforms, each eight cars long, that serve the outer tracks of the four-track Harlem Line.

The Harlem Line is an 82-mile (132 km) commuter rail line owned and operated by the Metro-North Railroad in the U.S. state of New York. It runs north from New York City to Wassaic, in eastern Dutchess County. The lower 53 miles (85 km) from Grand Central Terminal to Southeast, in Putnam County, is electrified with a third rail and has at least two tracks. The section north of Southeast is a non-electrified single-track line served by diesel locomotives. Before the renaming of the line in 1983, it eventually became the Harlem Division of the New York Central Railroad. The diesel trains usually run as a shuttle on the northern end of the line, except for rush-hour express trains in the peak direction.