DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA
HiSeq sequencers at SciLIfeLab in Uppsala, Sweden. The photo was taken during the excursion of SLU course PNS0169 in March 2019.
A schematic view of primers and target region, demonstrated on 16S rRNA gene in Pseudomonas. As primers, one typically selects short conserved sequences with low variability, which can thus amplify most or all species in the chosen target group. The primers are used to amplify a highly variable target region in between the two primers, which is then used for species discrimination. Modified from »Variable Copy Number, Intra-Genomic Heterogeneities and Lateral Transfers of the 16S rRNA Gene in Pseudomonas« by Bodilis, Josselin; Nsigue-Meilo, Sandrine; Besaury, Ludovic; Quillet, Laurent, used under CC BY, available from: