The Bratislava Zoo is a zoo in Bratislava, Slovakia. It is located in the area of Mlynská dolina in the borough Karlova Ves. Opened in 1960, it is the second oldest zoo in Slovakia. The zoo covers an area of 96 hectares, out of which 35 hectares is open to the public. The zoo receives on average around 300,000 visitors annually.

Karlova Ves is a borough in the city of Bratislava, the capital of Slovakia. It is located in the western part of the city close to the river Danube on the slopes of the Little Carpathians mountains and it is part of the Bratislava IV administrative district. A small, wine-making village for most of its history it was assimilated into Bratislava in the 1940s and in 1957, the construction of a large socialist panelák suburb started. Today, Karlova Ves has approximately 33,000 inhabitants and university dormitories in Mlynská dolina house an additional 15,000 students at the total area of 7874 meters squared.