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The entire wiki reimagined as a visual magazine with video discovery · Watch your interests come alive
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Bright-field microscopy
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Bright-field microscopy is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted white light, and contrast in the image is caused by attenuation of the
An example bright-field micrograph. This image shows a cross-section of the vascular tissue in a plant stem.
An example bright-field micrograph. This image shows a cross-section of the vascular tissue in a plant stem.
The oldest published image known to have been made with a microscope: bees by Francesco Stelluti, 1630
The oldest published image known to have been made with a microscope: bees by Francesco Stelluti, 1630
Loupe binoculaire p 1030891
Loupe binoculaire p 1030891
Phase-contrast microscopy
Videos
Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are
Bright-field microscopy
…contrast comes from the rotation of polarized light through the sample Dark-field illumination, sample contrast comes from light scattered by the sample Phase-contrast illumination, sample contrast comes from interference of different path lengths of light through the sample Enhancements Reducing or increasing the…
A phase-contrast microscope
A phase-contrast microscope
The same cells imaged with traditional bright-field microscopy (left), and with phase-contrast microscopy (right)
The same cells imaged with traditional bright-field microscopy (left), and with phase-contrast microscopy (right)
S. cerevisiae cells imaged by DIC microscopy
S. cerevisiae cells imaged by DIC microscopy
A quantitative phase-contrast microscopy image of cells in culture. The height and color of an image point correspond to the optical thickness, which
A quantitative phase-contrast microscopy image of cells in culture. The height and color of an image point correspond to the optical thickness, which only depends on the object's thickness and the relative refractive index. The volume of an object can thus be determined when the difference in refractive index between the object and the surrounding media is known.