The Brooker Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the CSX S Line at Wannee Junction in Starke to Newberry for a total of 39.6 miles.

Newberry is a city located in the southwest corner of Alachua County, Florida, United States. The population was 7,342 as of the 2020 Census, up from 4,950 at the 2010 census. It is part of the Gaines